Anti-Fractin (fragment of actin) Antibody

Product Name

Anti-Fractin (fragment of actin) Antibody

View all Lire1 Antibodies

SKU/Catalog Number

AT003

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Fractin (fragment of actin) Antibody (Catalog # AT003). Tested in WB, IHC, ICC applications. This antibody reacts with Human, Rat.

Storage & Handling

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Fractin (fragment of actin) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # AT003)

Host

Rabbit

Contents

Neat Serum

Clonality

Polyclonal

Clone Number

235-1

Isotype

IgG

Immunogen

Synthetic peptide corresponding to amino acid residues from the C-terminal region of the 32 kDa actin fragment. Immunogen species is Human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

AT003 is reactive to Lire1 in Human, Rat

Applications

AT003 is guaranteed for IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

41.226kDa

Background of Lire1

Fractin (fragment of actin) is a caspase-specific cleavage product of actin and serves as a novel marker of apoptosis-related events. The antibody has been shown to detect the processes and cell bodies of degenerating neurons and plaque-associated microglia in Alzheimer’s disease (Yang et al., 1998). It has recently been reported that Fractin may have a functional role in apoptotic signaling in oligodendrocytes (Schulz, R., et al., Glia, 2009, in press).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
IHC: 1:50-1:1000
ICC: 1:100-1:500

Validation Images & Assay Conditions

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Western blot of colchicine treated Sy5y cell lysate showing specific immunolabeling of the ~32 kDa cleaved actin fragment (fractin).

Gene/Protein Information For Lire1 (Source: Uniprot.org, NCBI)

Alternative Names

LINE-1 retrotransposable element ORF1 protein

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